| 郭佳雨,南丽丽,王利群,何蓉,任孟雨,马香香,田戈.三十烷醇对干旱胁迫下红豆草碳氮代谢及内源激素的影响[J].干旱地区农业研究,2026,(1):53~61 |
| 三十烷醇对干旱胁迫下红豆草碳氮代谢及内源激素的影响 |
| Effects of triacontanol on carbon\|nitrogen metabolism and endogenous hormones in sainfoin under drought stress |
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| DOI：10.7606/j.issn.1000-7601.2026.01.06 |
| 中文关键词: 三十烷醇 红豆草 干旱胁迫 碳氮代谢 内源激素 |
| 英文关键词:triacontanol sainfoin drought stress carbon\|nitrogen metabolism endogenous hormones |
| 基金项目:国家自然科学基金（32360339）；财政部和农业农村部：国家现代农业产业技术体系（CARS-34） |
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| 中文摘要: |
| 为探讨三十烷醇（Triacontanol，TA）对干旱胁迫红豆草（Onobrychis viciifolia）幼苗碳氮代谢及内源激素的影响，采用营养液砂培法，以地方品种甘肃红豆草GS、耐旱红豆草新品系P1及不耐旱红豆草2049为材料，用前期筛选的干旱敏感浓度-1.0 MPa PEG-6000及最佳TA浓度（10.0 μmol·L-1）对红豆草进行处理，即Hoagland营养液（CK）、-1.0 MPa PEG-6000干旱胁迫处理（T1）和干旱胁迫下喷施10.0 μmol·L-1的TA处理（T2），测定红豆草苗期地上部和地下部碳氮代谢和内源激素含量。结果表明，PEG-6000处理降低了供试红豆草地上部和地下部的硝态氮、氨态氮、游离氨基酸、生长素、玉米素和赤霉素含量，而T2处理后上述各指标的值均有所上升。相较于T1处理，T2处理下供试红豆草GS、P1和2049的地上部硝态氮、氨态氮、游离氨基酸、生长素、玉米素、赤霉素含量增幅分别为1.1%~3.0%、1.3%~10.4%、3.6%~15.0%、13.3%~20.2%、14.6%~24.4%、5.3%~11.5%，地下部相应指标含量增幅分别为1.9%~7.1%、1.5%~4.2%、0.8%~4.6%、4.0%~7.7%、11.9%~14.7%和4.0%~14.3%。而在T1处理下供试红豆草地上部、地下部的可溶性蛋白、可溶性糖、淀粉、蔗糖、果糖、葡萄糖、脱落酸含量和碳氮比相较于CK均上升，在T2处理后下降；T2与T1处理相比，GS、P1和2049的地上部上述各指标降幅分别为2.6%~9.8%、11.3%~41.4%、13.6%~59.8%、2.3%~38.6%、4.1%~7.8%、27.7%~31.5%、12.2%~15.4%、37.4%~41.9%，地下部相应指标降幅分别为5.8%~7.4%、34.0%~42.8%、13.6%~53.1%、27.8%~34.7%、4.8%~11.3%、15.9%~32.2%、8.6%~11.1%和23.0%~48.0%；且喷施TA所表现出的缓解效果为2049＞P1＞GS。可见喷施TA有助于维持干旱胁迫下红豆草碳水化合物的合成和植物生长激素的平衡，进而缓解干旱对红豆草生长发育的不利影响。 |
| 英文摘要: |
| To investigate the effects of triacontanol (TA) on carbon and nitrogen metabolism and endogenous hormones in sainfoin (Onobrychis viciifolia) seedlings under drought stress, a sand culture method with nutrient solution was employed. Using Gansu sainfoin (local vatiety, GS), new line P1 (drought\|resistant), and line 2049 (drought\|sensitive) as test materials, treatments were applied based on the pre\|screened drought\|sensitive concentration of -1.0 MPa PEG-6000 and the optimal TA concentration (10.0 μmol·L-1). The treatments included Hoagland nutrient solution (CK), -1.0 MPa PEG-6000 drought stress treatment (T1), and drought stress with 10.0 μmol·L-1 TA spray treatment (T2). Carbon and nitrogen metabolism indicators and endogenous hormone contents in the aboveground and underground parts of sainfoin seedlings were measured. The results showed that PEG-6000 inhibited nitrate nitrogen, ammonium nitrogen, free amino acids, auxin, zeatin, and gibberellin contents in both the aboveground and underground parts of the tested sainfoin. However, the values of these indicators increased under T2 treatment. Compared with T1, the T2 treatment increased nitrate nitrogen in the aboveground parts of GS, P1, and 2049 by 1.1%-3.0%, ammonium nitrogen by 1.3%-10.4%, free amino acids by 3.6%-15.0%, auxin by 13.3%-20.2%, zeatin by 14.6%-24.4%, and gibberellin by 5.3%-11.5%. In the underground parts, the corresponding increases were 1.9%-7.1%, 1.5%-4.2%, 0.8%-4.6%, 4.0%-7.7%, 11.9%-14.7%, and 4.0%-14.3%, respectively. Under T1 treatment, soluble protein, soluble sugar, starch, sucrose, fructose, glucose, abscisic acid content, and the C/N ratio in both the aboveground and underground parts of the tested sainfoin increased compared with CK, but decreased after T2 treatment. Compared with T1, the T2 treatment resulted in decreases in the aboveground parts of GS, P1, and 2049 as follows: soluble protein by 2.6%-9.8%, soluble sugar by 11.3%-41.4%, starch by 13.6%-59.8%, sucrose by 2.3%-38.6%, fructose by 4.1%-7.8%, glucose by 27.7%-31.5%, abscisic acid content by 12.2%-15.4%, and C/N ratio by 37.4%-41.9%. In the underground parts, the corresponding decreases were 5.8%-7.4%, 34.0%-42.8%, 13.6%-53.1%, 27.8%-34.7%, 4.8%-11.3%, 15.9%-32.2%, 8.6%-11.1%, and 23.0%-48.0%, respectively. The mitigation effect of TA spray followed the order: 2049 > P1 > GS. These findings indicate that TA application helps maintain carbohydrate synthesis and the balance of plant growth hormones in sainfoin under drought stress, thereby alleviating the adverse effects of drought on its growth and development. |
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