丛枝菌根真菌对地黄光合效率及生物量分配的影响

芦晶晶; 张梦汉; 石兆勇; 苑明莉; 李琰; 高佳凯

芦晶晶,张梦汉,石兆勇,苑明莉,李琰,高佳凯.丛枝菌根真菌对地黄光合效率及生物量分配的影响[J].干旱地区农业研究,2026,(1):116~125

丛枝菌根真菌对地黄光合效率及生物量分配的影响

Effects of arbuscular mycorrhizal fungi on photosynthetic efficiency and biomass allocation of Rehmannia glutinosa

DOI：10.7606/j.issn.1000-7601.2026.01.12

中文关键词: 地黄丛枝菌根真菌光合效率气体交换生物量分配

英文关键词:Rehmannia glutinosa arbuscular mycorrhizal fungi photosynthetic efficiency gas exchange biomass allocation

基金项目:国家自然科学基金（32171620）；河南省自然科学基金（242300420598）

作者	单位
芦晶晶	河南科技大学农学院,河南洛阳 471000
张梦汉	河南周口国家农高区现代农业产业研究院,河南周口 477100
石兆勇	河南科技大学农学院,河南洛阳 471000；河南省乡村人居环境工程中心,河南洛阳 471000；洛阳市共生微生物与绿色发展重点实验室,河南洛阳 471000
苑明莉	河南科技大学农学院,河南洛阳 471000
李琰	河南科技大学农学院,河南洛阳 471000
高佳凯	河南科技大学农学院,河南洛阳 471000；河南省乡村人居环境工程中心,河南洛阳 471000；洛阳市共生微生物与绿色发展重点实验室,河南洛阳 471000

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中文摘要:

为探究丛枝菌根真菌（AMF）对地黄光合效率及其生物量分配的影响，利用灭菌土壤接种AMF进行地黄盆栽试验，于生长60 d后测定地黄光合参数和荧光参数等指标。结果表明：接种AMF的地黄总生物量显著高于未接种对照（CK），且接种Rhizophagus intraradices+Gigaspora ramisporophora（RG）处理（6.62 g）＞接种G. ramisporophora（G）处理（4.96 g）＞接种R. intraradices（R）处理（3.32 g）；RG处理地黄的地下部鲜质量和干质量占比分别为69.98%和66.77%，最有利于养分向地下部药用部位运输积累。AMF处理下地黄具有更高的光合效率，其光合作用相关的CO₂吸收与H₂O蒸腾的气体交换能力显著提高，与CK相比，其气孔导度（G_s）、叶片CO₂导度（G_c）、蒸腾速率（T_r）和胞间CO₂浓度（C_i）分别增加65.0%~107.0%、125.86%~181.03%、32.0%~52.0%和12.19%~38.51%，叶片内部水汽浓度（C_H₂O,i）降低6.53%~10.18%，但代表光化学性能的参数无显著变化。综上可知，接种AMF可提高地黄叶片CO₂和H₂O的气体交换能力，强化CO₂和H₂O相关气体交换通道，促进CO₂气体的向内和H₂O的向外转移，进而提升地黄的净光合速率，最终增加了地上部及地下部的生物量，促进养分向地黄地下部药用部位的运输及积累，两种菌剂互作的效果最好。

英文摘要:

To explore the effects of arbuscular mycorrhizal fungi (AMF) on the photosynthetic efficiency and biomass allocation of Rehmannia glutinosa, a pot experiment was conducted using sterilized soil inoculated with AMF to grow R. glutinosa, and the photosynthetic parameters and fluorescence parameters were measured after 60 days of growth. The results showed that the total biomass of R. glutinosa inoculated with AMF was significantly higher than that of uninoculated R. glutinosa, with Rhizophagus intraradices + Gigaspora ramisporophora (RG) treatment (6.62 g) > G. ramisporophora (G) treatment (4.96 g) > R. intraradices (R) treatment (3.32 g). The proportion of fresh mass and dry mass of underground biomass of R. glutinosa under RG treatment was 69.98% and 66.77%, respectively, which was most conducive to the transportation and accumulation of nutrients to the underground medicinal parts. R. glutinosa inoculated with AMF showed a higher photosynthetic efficiency, and its gas exchange capacity related to CO₂ absorption and H₂O transpiration during photosynthesis was significantly enhanced. Compared with CK, stomatal conductance (G_s), leaf CO₂ conductance (G_c), transpiration rate (T_r), and intercellular CO₂ concentration (C_i) increased by 65.0% to 107.0%, 125.86% to 181.03%, 32.0% to 52.0%, and 12.19% to 38.51%, respectively. The internal water vapor concentration (C_H₂O,i) in the leaves decreased by 6.53% to 10.18 %, but the indicators representing the photochemical performance parameters did not change significantly. In summary, inoculation with AMF can enhance the CO₂ and H₂O gas exchange capacity in the leaves of R. glutinosa, strengthen the related gas exchange pathways, promote CO₂ influx and H₂O efflux, increase the net photosynthetic rate, and ultimately boost the biomass of the aboveground and underground biomass and facilitate the transport and accumulation of nutrients to the underground medicinal parts of R. glutinosa. The combined inoculation of two AMF strains showed the greatest effect.

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