| 王旭,刘媛媛,顾俊,王娅蒙,陈建茹,张桦,王波.过表达梭梭HaFT-1基因拟南芥的高温和干旱交叉胁迫适应性分析[J].干旱地区农业研究,2026,(2):168~179 |
| 过表达梭梭HaFT-1基因拟南芥的高温和干旱交叉胁迫适应性分析 |
| Adaptation analysis of Arabidopsis thaliana overexpressing Haloxylon ammodendron HaFT-1 gene under heat and drought cross\|stress |
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| DOI：10.7606/j.issn.1000-7601.2026.02.16 |
| 中文关键词: 梭梭 HaFT-1基因 拟南芥 过表达 高温-干旱交叉胁迫 |
| 英文关键词:Haloxylon ammodendron HaFT-1 gene Arabidopsis thaliana overexpression heat and drought cross\|stress |
| 基金项目:国家自然科学基金(32360079) |
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| 中文摘要: |
| 为探究HaFT-1蛋白在高温与干旱交叉胁迫耐受中的功能，以梭梭幼苗和HaFT-1过表达拟南芥为材料，通过qRT-PCR、生理指标测定和细胞染色等方法，分析了高温锻炼对HaFT-1基因表达的影响以及该基因在交叉胁迫响应中的作用。结果表明：高温锻炼后，梭梭根中HaFT-1基因在次级干旱胁迫下的相对表达量(3.81倍)显著高于单一干旱处理(2.92倍)；在拟南芥中，过表达HaFT-1基因株系在高温锻炼后干旱胁迫下的幼苗存活率(87.0%)高于单一干旱处理(81.7%)，且均高于野生型(约76.7%)；同时，转基因株系在次级干旱下的叶片细胞死亡率(41.7%)低于单一干旱(49.3%)，且低于野生型(47.7%)；生理检测显示，转基因株系在胁迫后抗氧化酶活性更高，其中过氧化氢酶(CAT)在高温锻炼后干旱处理下较野生型酶活性的提升达显著水平（P<0.05）；基因表达分析发现，高温锻炼后干旱胁迫下，转基因株系中光合作用、脯氨酸合成及胁迫响应相关基因的表达量较野生型上调2~200倍。以上结果说明，HaFT-1蛋白通过增强抗氧化防御、维持活性氧稳态并调控多重胁迫应答通路，正向调节植物对高温与干旱交叉胁迫的耐受性。 |
| 英文摘要: |
| To explore the function of the HaFT-1 protein in the cross\|stress tolerance of heat and drought stress, Haloxylon ammodendron seedlings and HaFT-1 overexpressing Arabidopsis thaliana were used as materials. The effects of heat acclimation on the expression of HaFT-1 and the role of this gene in cross\|stress response were analyzed through qRT-PCR, physiological indicator measurements, and cell staining methods. The results showed that after heat acclimation, the relative expression of the HaFT-1 gene in H. ammodendron roots under subsequent drought stress (3.81-fold) was significantly higher than under single drought treatment (2.92-fold). In A. thaliana, the seedling survival rate of HaFT-1 overexpressing lines under drought stress after heat acclimation (87.0%) was higher than under single drought treatment (81.7%), and both were higher than that of the wild type (approximately 76.7%). Additionally, leaf cell mortality in transgenic lines under subsequent drought stress (41.7%) was lower than under single drought stress (49.3%) and also lower than that of the wild type (47.7%). Physiological assays showed that transgenic lines exhibited higher antioxidant enzyme activities after stress, with the catalase (CAT) activity significantly increased under drought treatment after heat acclimation compared to the wild type (P<0.05). Gene expression analysis revealed that, under drought stress following heat acclimation, the expression levels of genes related to photosynthesis, proline synthesis, and stress response in transgenic lines were upregulated by 2- to 200-fold compared to the wild type. These results indicate that the HaFT-1 protein positively regulates plant tolerance to heat and drought cross\|stress by enhancing antioxidant defense, maintaining reactive oxygen species homeostasis, and modulating multiple stress response pathways. |
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