| 郭浩杰,王成,杨馥熔,杜冰,孟超敏.谷子SPX基因家族鉴定及响应低磷胁迫的表达模式分析[J].干旱地区农业研究,2026,(2):180~191 |
| 谷子SPX基因家族鉴定及响应低磷胁迫的表达模式分析 |
| Identification of SPX gene family in foxtail millet and analysis of its expression pattern in response to low phosphorus stress |
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| DOI：10.7606/j.issn.1000-7601.2026.02.17 |
| 中文关键词: 谷子 SPX基因家族 低磷胁迫 表达分析 |
| 英文关键词:Setaria italica L. SPX gene family low phosphorus stress expression analysis |
| 基金项目:河南省科技攻关项目（242102110287）；河南省产业科技特派员项目（2018HNCYTPY01）；河南省旱地绿色智慧农业特色骨干学科群建设项目（17100001） |
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| 中文摘要: |
| 通过生物信息学方法对谷子SPX基因家族进行全基因组鉴定，结合实时荧光定量PCR（RT-qPCR）技术，系统分析该基因家族成员在谷子不同组织（根、茎、叶、穗）及低磷胁迫下的表达模式，以期为谷子SPX基因家族功能研究及遗传改良提供理论依据。结果表明：谷子SiSPX基因家族中共包含15个SPX成员（SiSPX1~SiSPX15），这些成员不均匀分布于6条染色体上，且存在基因片段复制现象；理化性质分析表明，其编码的氨基酸序列长度为252~849 aa，分子量为28.22~97.22 KDa，等电点介于5.24~9.84；系统发育分析显示，谷子SPX基因家族可分为4个亚族（SPX、SPX-EXS、SPX-MFS和SPX-RING），同一亚族成员具有相似的基因结构与保守基序；启动子顺式作用元件分析表明，所有SPX基因家族成员的启动子序列均含有与植物生长发育、激素响应、非生物胁迫响应相关的顺式元件。RT-qPCR结果显示，SiSPX基因表达具有明显的组织特异性，其成员在谷子不同组织中均有表达，其中SiSPX2/3/4/9/14/15在根中表达量较高，SiSPX5/7/8/10/13在茎中高表达，SiSPX1/6/11在叶中表达量较高。低磷胁迫下的分析结果表明，有13个基因的表达响应低磷胁迫，且在不同处理时期的表达模式存在差异，SiSPX9在处理12 h时表达水平最高，SiSPX6在72 h时表达水平最高，SiSPX10在4 h时表达水平最高，且均与正常供磷处理存在极显著差异。 |
| 英文摘要: |
| The whole\|genome identification and analysis of the SPX gene family in foxtail millet were carried out by bioinformatics methods. Combined with quantitative real\|time PCR (RT-qPCR) technology, the expression patterns of SPX gene family members in different tissues (roots, stems, leaves, and spikes) of foxtail millet and under low phosphorus stress were systematically analyzed to provide a theoretical basis for functional study and genetic improvement of the SPX gene family in foxtail millet. The results showed that there were 15 SPX members (SiSPX1-SiSPX15) in the SiSPX gene family of foxtail millet, which were unevenly distributed on 6 chromosomes, and there was gene fragment replication. Physical and chemical property analysis showed that the length of the amino acid sequence was between 252 and 849 aa, the molecular weight was between 28.22 and 97.22 KDa, and the isoelectric point was between 5.24 and 9.84. Phylogenetic analysis showed that the millet SPX gene family could be divided into four subfamilies (SPX, SPX-EXS, SPX-MFS, and SPX-RING). The members of the same subfamily had similar gene structures and conserved motifs. The analysis of promoter cis\|acting elements showed that promoter sequences of all members of the SPX gene family contain cis\|elements related to plant growth and develop\|ment, hormone response, and abiotic stress response. The results of RT-qPCR showed that the expression of the SiSPX gene had obvious tissue specificity, and its members were expressed in different tissues. Among them, SiSPX2/3/4/9/14/15 were highly expressed in roots, SiSPX5/7/8/10/13 were highly expressed in stems, and SiSPX1/6/11 were highly expressed in leaves. The analysis results under low phosphorus stress showed that the expression of 13 genes responded to low phosphorus stress and had different expression patterns at different treatment stages. SiSPX9 showed the highest expression level at 12 hours, SiSPX6 had the highest expression level at 72 hours, and SiSPX10 had the highest expression level at 4 hours. The expression of the above genes all showed highly significant differences compared to normal phosphorus\|supply treatment. |
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