| 曹海艳,张子彤,宋南楠,乔硕儿,郝雪峰,金竹萍.谷子SiBBX基因家族的鉴定及表达分析[J].干旱地区农业研究,2026,(4):34~44 |
| 谷子SiBBX基因家族的鉴定及表达分析 |
| Genome\|wide identification and expression analysis of the SiBBX gene family in foxtail millet (Setaria italica L.) |
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| DOI：10.7606/j.issn.1000-7601.2026.04.04 |
| 中文关键词: 谷子 SiBBX基因家族 生物信息学分析 非生物胁迫 激素响应 表达分析 |
| 英文关键词:Setaria italica L. SiBBX gene family bioinformatics analysis abiotic stress phytohormone response expression analysis |
| 基金项目:山西省基础研究计划资助项目（202403021212063，202503021211222）；山西大学杏花村学院开放基金项目：制曲大麦花青素积累的影响因素研究（XCSXU-KF-202401） |
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| 中文摘要: |
| 为解析谷子BBX转录因子家族的进化特征与逆境响应功能，采用生物信息学方法鉴定谷子SiBBX基因家族成员，分析其编码蛋白理化性质、系统进化关系、共线性、基因结构及顺式作用元件组成，同时利用qRT-PCR技术检测该基因家族在不同非生物胁迫及激素处理下的表达模式特征。结果显示，谷子全基因组中鉴定获得17个SiBBX基因，不均匀分布于7条染色体；其编码蛋白氨基酸长度为218~465 aa，分子量22.29~50.22 kDa，等电点4.79~8.00，均为不稳定亲水性蛋白，且亚细胞主要定位于细胞核或叶绿体。系统进化树将SiBBX蛋白划分为7个亚家族，同一亚家族成员具有高度保守的基序组成和结构域特征；种内共线性检测到4对同源复制基因，Ka/Ks值均小于1，证明SiBBX基因家族进化受纯化选择约束。启动子分析显示，SiBBX基因家族启动子区域富集非生物胁迫、激素、生长发育、生物防御、次生代谢5大类调控元件，其中光响应、脱落酸应答元件为家族共有保守元件。qRT-PCR结果表明，候选SiBBX基因在非生物胁迫和激素处理下表现出差异表达模式，其中SiBBX3、SiBBX9和SiBBX12呈现相对特异的响应特征。综上，SiBBX基因在谷子进化中结构保守，可能广泛参与谷子非生物胁迫响应及激素信号相关调控过程。 |
| 英文摘要: |
| To elucidate the evolutionary characteristics and functions in stress responses of the BBX transcription factor family in foxtail millet, the members of the SiBBX gene family were systematically identified using bioinformatics approaches,and the phylogenetic relationships, synteny, gene structures, and cis\|acting element compositions of these SiBBX genes, as well as the physicochemical properties of the encoded proteins, were investigated. Meanwhile, the expression profiles of this gene family under various abiotic stress and phytohormone treatments were examined via quantitative real\|time PCR (qRT-PCR). A total of 17 SiBBX genes were identified in the foxtail millet genome, unevenly distributed across seven chromosomes. The encoded proteins ranged from 218 to 465 amino acids in length, with molecular weights of 22.29 to 50.22 kDa and isoelectric points between 4.79 and 8.00; moreover, all of them were classified as unstable hydrophilic proteins and were predominantly predicted to localize to the nucleus or chloroplast. Phylogenetic analysis partitioned the SiBBX proteins into seven subfamilies, with members within the same subfamily exhibiting highly conserved motif compositions and domain features. Intraspecific synteny analysis detected four pairs of duplicate genes, all with Ka/Ks ratios below 1, indicating that the evolution of the SiBBX gene family is constrained by purifying selection. Promoter analysis revealed that the promoter regions of SiBBX genes are enriched in five major categories of regulatory elements, namely response to abiotic stresses, hormones, growth and development, biological defense, and secondary metabolism, among which light\|responsive and abscisic acid\|responsive elements were conserved across the family. qRT-PCR results demonstrated that the candidate SiBBX genes exhibited differential expression patterns in response to abiotic stresses and hormone treatments, with SiBBX3, SiBBX9, and SiBBX12 showing relatively specific responsiveness. Collectively, these findings suggest that SiBBX genes are structurally conserved during foxtail millet evolution and may be broadly involved in abiotic stress responses and hormone\|related regulatory processes in this crop. |
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