| The experiment was conducted to study the transpiration of de-root shoot and leaves in vitro treated with phosphoric acid buffer and artificial solution(AS) buffer. The results showed that pH treatment could significantly influence the transpiration. Different buffer, and even the same buffer but with different pH value, had different effect on transpiration. The AS buffer influenced the relative water content(RWC) of maize leaves in vitro largely, and the RWC was the lowest under pH 7.5 treatment. |