| Study on the optimal selective culture medium in Populus xeuramericana ‘Neva’ transformation |
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| DOI：10.7606/j.issn.1000-7601.2005.03.36 |
| Key Words: culture medium NaCl Kanamycin Populus x euramericana 'Neva&apos |
| Author Name | Affiliation | | CHEN Juan | College of Life Science,Northwest Sci-Tech University of Agriculture and Forestry,Yangling Shaanxi 712100,China | | GUO Shan-li | Key Laboratory of Plant Stress Research,Shandong Normal University,J inan Shandong 250014,China | | LV Jin-yin | College of Life Science,Northwest Sci-Tech University of Agriculture and Forestry,Yangling Shaanxi 712100,China | | ZHANG Hui | Key Laboratory of Plant Stress Research,Shandong Normal University,J inan Shandong 250014,China |
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| Abstract: |
| Different concentrations of NaCl and Kanamycin were used for studying on selection of transgenic Poplar plantlets. Through comparing the differentiation rate and growth potential of transgenic plants and control, then transgenic plant proved by PCR analysis, we acquired optimal selective medium in our transformation system. NaCl can't be used for selective marker. Kanamycin can be used for selective marker in our system. And the optimal selective medium was modified MS with BA 0.5 mg/L, NAA 0.1 mg/L, ceftriaxone sodium 200 mg/L and Kanamycin 100 mg/L. |
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