| Oat （Avena sativa L.） exogenous DNA introduction into common wheat and PAPD analysis |
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| DOI：10.7606/j.issn.1000-7601.2006.03.21 |
| Key Words: oat (Avena sativa L.) exogenous DNA introduction pollen tube pathway ningchun 4 spring wheat RAPD |
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| Abstract: |
| 12 variant strains have been developed form pollen tube pathway method by taking oat (Avena sativa L.) and common wheat(Ningchun 4) as donor and recipient.The major agronomic traits of variant strains such as the flagleaf area,spike length,grain number of main spike,grain weight of main spike and weight of thousand grains were significantly exceeded of Ningchun 4.RAPD analysis with 68 random primers was performed.Products of each primer were detected to have polymorphism among oat,Ningchun 4 and variant strains.The specific DNA fragments of donor have been found in genome of progenies,which didn't exist in the original recipient. Oat total DNA fragments can insert into recipient through pollen tube pathway and perform phenotypic expression can be inherited in progenies.This has been directly verified through technique of molecular level of DNA. |
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