| Effects of stress on the indirubin content of Isatis indigotica Fort．seedlings |
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| DOI：10.7606/j.issn.1000-7601.2006.03.24 |
| Key Words: Isatis indigotica Fort. indirubin stress HPLC |
| Author Name | Affiliation | | DUAN Fei | College of Life Science Shaanxi Normal University, Xi’an 710062,China | | YANG Jian-xiong | College of Life Science Shaanxi Normal University, Xi’an 710062,China | | ZHOU X-i kun | College of Life Science Shaanxi Normal University, Xi’an 710062,China | | MA Tian-li | College of Life Science Shaanxi Normal University, Xi’an 710062,China | | YU Jia-ning | College of Life Science Shaanxi Normal University, Xi’an 710062,China |
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| Abstract: |
| The seedlings of Isatis indigotica Fort.cultivated for 30 days in aseptic environment were treated with 250 mmol/LNaCl,natural drought method,20 mmol/L ABA,4℃ low temperature,respectively.The content of indirubin was extracted from Isatis indigotica Fort.seedlings at the indicated times and analyzed using HPLC method,the results showed that the content of indirubin could increased up to 59.05%～161.67% in salt,drought and ABA treatment during 12 h.The content of indirubin increased up to 141.4% under low temperature stress for 24 h.These implied that the content of indirubin in the Isatis indigotica Fort.seedlings could accumulate by appropriate stress and ABA treatment efficiently.The 250 mmol/L NaCl stress treatment had the most effect. |
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