| Establishment of the acceptor system for the gene transformation of Lilium Asiatic hybrids |
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| DOI：10.7606/j.issn.1000-7601.2007.01.46 |
| Key Words: Lilium Asiatic gene transformation acceptor system |
| Author Name | Affiliation | | LI Xiao-ling | College of Horticulture Northwest Sc-iTech University of Agriculture and Forestry,Yangling,Shaanxi 712100,China | | LIU Ya-li | College of Horticulture Northwest Sc-iTech University of Agriculture and Forestry,Yangling,Shaanxi 712100,China | | WANG Yue-jin | College of Horticulture Northwest Sc-iTech University of Agriculture and Forestry,Yangling,Shaanxi 712100,China | | HUA Zhi-rui | College of Horticulture Northwest Sc-iTech University of Agriculture and Forestry,Yangling,Shaanxi 712100,China | | CHEN Yuan | College of Horticulture Northwest Sc-iTech University of Agriculture and Forestry,Yangling,Shaanxi 712100,China |
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| Abstract: |
| Using scales and petioles of Lilium Asiatic hybrid as the study subject,the acceptor system for the gene transformation of Lilium Asiatic hybrids was established.The results showed that the medium suitable for inducing the scales to differentiate Adventitious buds was MS+6-BA 2.0 mg/L+NAA 0.2(mg/L,) the medium suitable for small bulb induction was MS+IBA 0.5 mg/L+Sucrose 70 g/L, the medium suitable for the leaves of the test-tube plantlets to differentiate adventitious buds was MS+TDZ 1.0 mg/L+NAA 0.2(mg/L,) and the medium suitable for the scale leaves of the test-tube plantlets to differentiate adventitious buds was MS+6-BA 2.0 mg/L+2,4-D 0.1 mg/L.The concentrations of the antibiotics for different parts of the test-tube plantlets in their cultures were determined,the concentration of Kanamycin for the petioles was 80 mg/L and for the scales 115 mg/L. |
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