The physiological responses of tobacco plantlets in vitro by salt stress |
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DOI:10.7606/j.issn.1000-7601.2007.04.47 |
Key Words: tobacco plantlets in vitro salt stress physiological effect |
Author Name | Affiliation | DU Xi-mei | College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China | ZHANG Jun-lian | College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China;Gansu Key Laboratory of Crop Genetics & Germplasm Enhancement, Lanzhou, Gansu 730070, China | WANG Di | College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China;Gansu Key Laboratory of Crop Genetics & Germplasm Enhancement, Lanzhou, Gansu 730070, China | HUANG Peng | College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China | CUI Yan-sen | College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China | WANG Li | College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China | ZHANG Tian-yu | College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China |
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Abstract: |
The tobacco plantlets in Vitro were treated with different concentrations of NaCl in 20 days. The result indicated that the plant growth was retarded steadily with the increase of salt concentration; In this case, the content of K+ reduced and the content of Na+ increased in root, stem and leaf; In leaf, content of chlorophyll reduced, but the content of malonaldehyde(MDA), membrane permeability and proline increased, moreover the content of proline showed significant positive correlation with the content of malonaldehyde(MDA) and membrane permeability. It showed that the accumulative content of praline could reflect the tobacco plantlets' hurt degree. The study indicated that either the hurt of ion, the increase of membrane permeability or the reduction of chlorophyll affected the growth of tobacco plantlets in vitro of salt treatment. |
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