The physiological responses of tobacco plantlets in vitro by salt stress
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DOI:10.7606/j.issn.1000-7601.2007.04.47
Key Words: tobacco plantlets in vitro  salt stress  physiological effect
Author NameAffiliation
DU Xi-mei College of Agronomy, Gansu Agricultural University Lanzhou, Gansu 730070 China 
ZHANG Jun-lian College of Agronomy, Gansu Agricultural University Lanzhou, Gansu 730070 ChinaGansu Key Laboratory of Crop Genetics & Germplasm Enhancement, Lanzhou, Gansu 730070, China 
WANG Di College of Agronomy, Gansu Agricultural University Lanzhou, Gansu 730070 ChinaGansu Key Laboratory of Crop Genetics & Germplasm Enhancement, Lanzhou, Gansu 730070, China 
HUANG Peng College of Agronomy, Gansu Agricultural University Lanzhou, Gansu 730070 China 
CUI Yan-sen College of Agronomy, Gansu Agricultural University Lanzhou, Gansu 730070 China 
WANG Li College of Agronomy, Gansu Agricultural University Lanzhou, Gansu 730070 China 
ZHANG Tian-yu College of Agronomy, Gansu Agricultural University Lanzhou, Gansu 730070 China 
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Abstract:
      The tobacco plantlets in Vitro were treated with different concentrations of NaCl in 20 days. The result indicated that the plant growth was retarded steadily with the increase of salt concentration; In this case, the content of K+ reduced and the content of Na+ increased in root, stem and leaf; In leaf, content of chlorophyll reduced, but the content of malonaldehyde(MDA), membrane permeability and proline increased, moreover the content of proline showed significant positive correlation with the content of malonaldehyde(MDA) and membrane permeability. It showed that the accumulative content of praline could reflect the tobacco plantlets' hurt degree. The study indicated that either the hurt of ion, the increase of membrane permeability or the reduction of chlorophyll affected the growth of tobacco plantlets in vitro of salt treatment.