| To indentify anti-coldness of B.napus L.varieties,temperature-controlling method by artificial climatic box is introduced to deal with 12 B.napus L.varieties from abroad under low temperature above zero for observance of different varieties' anti-coldness index variation.The results showed that total water content in leaves of all varieties decreased with the decrease of temperature,however,membrane relativepenetrat,malondialdehycle(MDA)content,soluble sugar and free proline content increased with the decrease of temperature.The varieties of Alice,Orkan,Hopper and Parade-2 were screened with high cold hardness,but Olsen and Embleme had lower cold hardness than the other varieties.Simple correlation analysis showed that membrane relative penetratio and soluble sugar content had the closest relations with the cold hardness of winter rape. |