| Determination of the total isoflavone content of chickpea seeds by three-wavelength UV spectrophotometry |
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| DOI：10.7606/j.issn.1000-7601.2007.06.20 |
| Key Words: Cicer Arietinum L. total isoflavones content three-wavelength determination |
| Author Name | Affiliation | | WU Min | State Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricμltural University,Nanjing,Jiangsu 210095,China；College of Agronomy Xinjiang Agricμltural University,Urumchi,Xinjiang 830052,China | | YUAN Jian | College of Food Science and Engineering Nanjing Finance and Economics University,Nanjing,Jiangsu 210003,China | | YU Tian | College of Agronomy Xinjiang Agricμltural University,Urumchi,Xinjiang 830052,China | | LI Yan | College of Agronomy Xinjiang Agricμltural University,Urumchi,Xinjiang 830052,China | | WANG Hong-ling | State Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricμltural University,Nanjing,Jiangsu 210095,China | | ZHANG Ju-song | College of Agronomy Xinjiang Agricμltural University,Urumchi,Xinjiang 830052,China | | MA Hao | State Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricμltural University,Nanjing,Jiangsu 210095,China |
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| Abstract: |
| The extraction and determination were optimized to build a determining method of the total isoflavone content of chickpea seeds by Three-wavelength UV spectrophotometry.The optimum extraction condition was: 0.3 g chickpea flour was extracted with 25 ml 70% alcohol density for 3 hours.The extracting temperature was 60℃.The isoflavone extracting rate was 86.5% at one time,while the total extraction rate of two times was 97.0%.The coefficient of variation(CV) of the accuracy experiment was 1.14%.The average recovery rate was 99.0% with CV=3.2%.The newly developed determining method is reliable,simple and accurate,and could avoid or eliminate the influence of the interfering components and the peak floating,and is suitable for the quality control of chickpea products. |
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