Effects of water stress on water potential and proline metabolism in leaves of sugarcane (Saccharum officinarum L.) at early growth stage |
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DOI:10.7606/j.issn.1000-7601.2008.01.37 |
Key Words: Saccharum officinarum L. polyethylene glycol(PEG) stress water potential proline metabolism |
Author Name | Affiliation | HUANG Chengmei | Guangxi Crop Genetic Improvement and Biotechnology Laboratory?Guangxi Academy of Agricultural Sciences,Nanning 530007, China;Agricultural College Guangxi University, Nanning 530005, China | YANG Litao | Agricultural College Guangxi University, Nanning 530005, China | JIANG Wen | Agricultural College Guangxi University, Nanning 530005, China;Biotechnology Research Institute Guangxi Academy of Agricultural Sciences, Nanning 530007, China | BI Liming | Agricultural College Guangxi University, Nanning 530005, China | LI Yangrui | Guangxi Crop Genetic Improvement and Biotechnology Laboratory?Guangxi Academy of Agricultural Sciences,Nanning 530007, China;Agricultural College Guangxi University, Nanning 530005, China |
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Abstract: |
Three sugarcane(Saccharum officinarum L.) varieties,‘Guitang119’,‘ROC16’ and ‘ROC22’,were used as experimental materials.The plants were treated with polyethylene glycol(PEG) 6000 at the early growth stage.Effects of water stress on water potential,proline content,activities of Δ1-pyrroline-5-carboxylate synthetase(P5CS),ornithine-δ-aminotransferase(δ-OAT) and proline dehydrogenase(ProDH) of leaves were studied.The results showed that,under the stress treatment,leaf water potential of three varieties decreased.And free proline content of ‘ROC16’ increased 1 d after the starting of treatment,‘GT119’ also increased 2 d after the starting of treatment,while there was no difference between treatment and control for the variety ‘ROC22’ during 12 d.The same results for the activity of P5CS were obtained after the stress treatment,the activity of ‘GT119’ and ‘ROC16’ increased after treatment,but ‘ROC22’ was contrary.For the activities of δ-OAT,there was no remarkable difference between the treatment and control.The proline dehydrogenase(ProDH) activity of ‘GT119’ and ‘ROC16’ increased at first then decreased after 7 d,‘ROC22’ increased 7 d after the starting of treatment. |
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