小麦淀粉合酶基因Ⅱ克隆及反义和RNAi载体构建

康国章; 岳彩凤; 官春云; 韩巧霞; 郭天财; 朱云集; 王永华

Molecular cloning of starch synthase Ⅱ partial sequences and construction of its antisense and RNAi expression vectors

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DOI：10.7606/j.issn.1000-7601.2008.03.22

Key Words: wheat （Triticum aestivum） starch synthase Ⅱ gene expression vectors

Author Name	Affiliation
KANG Guozhang	National Engineering Research Centre for Wheat Henan Agricultural University，Zhengzhou 450002， China
YUE Caifeng	National Engineering Research Centre for Wheat Henan Agricultural University，Zhengzhou 450002， China
GUAN Chunyun	National Engineering Research Centre for Wheat Henan Agricultural University，Zhengzhou 450002， China；Agronomy of Institute Hunan Agricultural University, Changsha 410012, China
HAN Qiaoxia	National Engineering Research Centre for Wheat Henan Agricultural University，Zhengzhou 450002， China
GUO Tiancai	National Engineering Research Centre for Wheat Henan Agricultural University，Zhengzhou 450002， China
ZHU Yunji	National Engineering Research Centre for Wheat Henan Agricultural University，Zhengzhou 450002， China
WANG Yonghua	National Engineering Research Centre for Wheat Henan Agricultural University，Zhengzhou 450002， China

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Abstract:

Starch synthase Ⅱ （SSⅡ） partial cDNA sequences （600bp）（GenBank No．EF221761） from grains of common wheat （ T riticum aestiv um Yujiao 2 cultuvar） was amplified by RT-PCR．The result demonstrated that the cloned SSⅡ gene sequences were99％ identified with the reported SSⅡ genes in Gen-Bank previously．In addition its antisense expression vector was constructed with pCMBIA1301 and RNAi vector was also constructed with pFGC5941．These constructed vectors will provide a good background to study the function of SSⅡ on biosynthesis of starch in wheat plants．