| Molecular cloning of starch synthase Ⅱ partial sequences and construction of its antisense and RNAi expression vectors |
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| DOI：10.7606/j.issn.1000-7601.2008.03.22 |
| Key Words: wheat （Triticum aestivum） starch synthase Ⅱ gene expression vectors |
| Author Name | Affiliation | | KANG Guozhang | National Engineering Research Centre for Wheat Henan Agricultural University,Zhengzhou 450002, China | | YUE Caifeng | National Engineering Research Centre for Wheat Henan Agricultural University,Zhengzhou 450002, China | | GUAN Chunyun | National Engineering Research Centre for Wheat Henan Agricultural University,Zhengzhou 450002, China；Agronomy of Institute Hunan Agricultural University, Changsha 410012, China | | HAN Qiaoxia | National Engineering Research Centre for Wheat Henan Agricultural University,Zhengzhou 450002, China | | GUO Tiancai | National Engineering Research Centre for Wheat Henan Agricultural University,Zhengzhou 450002, China | | ZHU Yunji | National Engineering Research Centre for Wheat Henan Agricultural University,Zhengzhou 450002, China | | WANG Yonghua | National Engineering Research Centre for Wheat Henan Agricultural University,Zhengzhou 450002, China |
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| Abstract: |
| Starch synthase Ⅱ （SSⅡ） partial cDNA sequences （600bp） （GenBank No．EF221761） from grains of common wheat （ T riticum aestiv um Yujiao 2 cultuvar） was amplified by RT-PCR．The result demonstrated that the cloned SSⅡ gene sequences were99％ identified with the reported SSⅡ genes in Gen-Bank previously．In addition its antisense expression vector was constructed with pCMBIA1301 and RNAi vector was also constructed with pFGC5941．These constructed vectors will provide a good background to study the function of SSⅡ on biosynthesis of starch in wheat plants． |
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