| The isolation effect of different basic culture media and pre-treatments on actinomycetes from alpine meadow was studied by using plate paint isolation methods.The results showed that: the best pre-treatments methods of the soil samples on actinomycete isolation is to treat with 120℃ for 1 hour,then to add 6% yeast extract and 0.05% SDS and vibrate at 40℃ for 30 minutes.It could promote actinomycetes spore sprouting to increase the quantity of actinomycetes and inhibit the development of other microorganisms.Meanwhile,the gause,s starch agar media added with K2Cr2O7(50 mg/L) and modified HV media were best culture medium for isolation of actinomycetes. |