| RFLP analysis on the impact of microbial diversity of dry land soils without nitrogenous fertilizer |
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| DOI：10.7606/j.issn.1000-7601.2009.02.37 |
| Key Words: nitrogenous fertilizer dry land soil bacterial diversity RFLP 16S rDNA |
| Author Name | Affiliation | | LI Yang | College of Life Sciences Northwest A＆F University,Yangling,Shaanxi 712100,China | | CEN Jian | College of Life Sciences Northwest A＆F University,Yangling,Shaanxi 712100,China | | WANG Baoli | College of Life Sciences Northwest A＆F University,Yangling,Shaanxi 712100,China；State Key Laboratory of Soil Erosion and Dryland Farming on Loess Plateau,Yangling,Shaanxi 712100,China | | QU Dong | College of Resources and Environment Northwest A＆F University,Yangling,Shaanxi 712100,China |
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| Abstract: |
| The impact of the absence of nitrogenous fertilizer on bacterial diversity in soil had been explored in this study．Total genome DNA of soil microorganism was extracted by a direct method from three soil samples of different fertilizing treatment which are CK without fertilizer F1 with proper fertilizer and F2 without nitrogenous fertilizer．The 16S rDNAs of the extracted DNA were amplified using bacterial universal primers and then set relevant clone libraries．Using PCR－RFLP based on restriction endonuclease Rsa I and Hha I simultaneously restriction endonuclease types of these samples were got as146 187and11after counting．All the data was analyzed by α diversity measurement indices which showed the identical order of diversity（ H′,Ds and Dg）and richness（dMa, R2 and E）between different treatment as F1＞CK＞F2．The coefficient of variation of indices λ, dMa, E and H′ between different soil samples is 56．96％～163．1％ especially for index λ with the greatest sensitivity had the biggest difference between three different soil samples suggesting that the bacterial diversity in soil was influenced seriously under absence of nitrogenous fertilizer and proper fertilizer was positive to them in contrast． |
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