Callus of Nitraria tangutorm Bobr.were used to investigate the effects of NaCl stress on growth, content of soluble protein, MDA, proline and activities of SOD, POD and CAT, in order to understand the physiological mechanism of Nitraria tangutorm Bobr.callus resistance to NaCl stress at cell level.The results indicated that free Proline and MDA content increased as NaCl concentration enhanced and stress time lengthened;the activities of Peroxidase (POD) first increased and then decreased as time lengthened under different NaCl concentration;the activities of superoxide dismutase (SOD) , catalase (CAT) , soluble protein and callus growth expressed increasing trend as time lengthened in low NaCl;but in high NaCl, they first increased and then decreased as time lengthened.It follows that N.tangutorm had a adaptability to salt environment, light salt stress is favorable for N.tangutorm callus to grow. |