Research on physiological and biochemical index changes of Nitraria tangutorm Bobr.callus under NaCl stress
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DOI:10.7606/j.issn.1000-7601.2009.03.38
Key Words: salt stress  Nitraria tangutorm Bobr.  callus  growth  physiological and biochemical index
Author NameAffiliation
FAN Xiaofeng Department of Life Sciences Longdong UniversityQingyang Gansu 745000 ChinaCollege of Life Sciences Northwest Normal University Lanzhou Gansu730070 China 
YANG Yingli College of Life Sciences Northwest Normal University Lanzhou Gansu730070 China 
CHENG Zhuanxia Department of Life Sciences Longdong UniversityQingyang Gansu 745000 China 
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Abstract:
      Callus of Nitraria tangutorm Bobr.were used to investigate the effects of NaCl stress on growth, content of soluble protein, MDA, proline and activities of SOD, POD and CAT, in order to understand the physiological mechanism of Nitraria tangutorm Bobr.callus resistance to NaCl stress at cell level.The results indicated that free Proline and MDA content increased as NaCl concentration enhanced and stress time lengthened;the activities of Peroxidase (POD) first increased and then decreased as time lengthened under different NaCl concentration;the activities of superoxide dismutase (SOD) , catalase (CAT) , soluble protein and callus growth expressed increasing trend as time lengthened in low NaCl;but in high NaCl, they first increased and then decreased as time lengthened.It follows that N.tangutorm had a adaptability to salt environment, light salt stress is favorable for N.tangutorm callus to grow.