Study on the growth and changes of several physiological indexes of potato plantlets in vitro under low phosphorus stress
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DOI:10.7606/j.issn.1000-7601.2009.05.33
Key Words: potato  plantlet in Vitro  low-phosphorus stress  physiological index
Author NameAffiliation
ZHAO Yingqin College of Agronomy Gansu Agricultural University, Lanzhou, Gansu 730070, China 
LIU Yuhui Gansu Key Laboratory of Crop Genetic &Germplasm Enhancement, Lanzhou, Gansu 730070, China 
WANG Li Gansu Key Laboratory of Crop Genetic &Germplasm Enhancement, Lanzhou, Gansu 730070, ChinaCollege of Life Sciences and Technology Gansu Agricultural University, Lanzhou, Gansu 730070, China 
YANG Hongyu College of Agronomy Gansu Agricultural University, Lanzhou, Gansu 730070, ChinaGansu Key Laboratory of Crop Genetic &Germplasm Enhancement, Lanzhou, Gansu 730070, China 
ZHANG Junlian College of Agronomy Gansu Agricultural University, Lanzhou, Gansu 730070, ChinaGansu Key Laboratory of Crop Genetic &Germplasm Enhancement, Lanzhou, Gansu 730070, China 
WANG Di College of Agronomy Gansu Agricultural University, Lanzhou, Gansu 730070, ChinaGansu Key Laboratory of Crop Genetic &Germplasm Enhancement, Lanzhou, Gansu 730070, China 
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Abstract:
      Potato plantlets in vitro were cultured on MS medium with full phosphorus level, 60% phosphorus level and 30% phosphorus level.Physiological indexes on 20 d and 40 d were determined and the results showed that the ability of new leaves and lateral root generation was promoted under low-p stress, which could also improve the root growth, increase the fresh weight of aboveground and underground slightly.Under the culture environment with 30% phosphorus, the relative permeability of cell membrane, soluble protein content and soluble sugar content increased and chlorophyll content decreased significantly compared with the control, and the significant difference was at the level of 5% or 1%.Under the treatment with 60% phosphorus, the changes of physiological indexes were the same as the control, and there was no obvious difference.The results demonstrated that it is suitable for potato plantlets in vitro growth on MS medium with 60% phosphorus.