| Salt-resistance identification of transformed Lilium with S6PDH gene |
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| DOI：10.7606/j.issn.1000-7601.2010.01.29 |
| Key Words: lily S6PDH gene salt resistance physiological indicator |
| Author Name | Affiliation | | HUA Zhirui | Biomedical Engineering Department, Shangluo University, Shangluo, Shaanxi 726000, China | | MA Fengwang | College of Horticulture, Northwest A & F University, Yangling, Shaanxi 712100, China | | LI Xiaoling | Biomedical Engineering Department, Shangluo University, Shangluo, Shaanxi 726000, China | | LIANG Dong | College of Horticulture, Northwest A & F University, Yangling, Shaanxi 712100, China |
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| Abstract: |
| Based on Agrobacterium-mediated S6PDH gene being delivered into Lilium longiflorum, by treatment of salt stress to the transformed plants and the control, some related physiological indicators were measured for a short period of time to lay foundation for improving resistant varieties of lily and studying the resistant mechanism of transgenic plants. The main findings are as following: With salt stress (10 g／L NaCl) to extend the time, leaves
of the conductivity and MDA of transformed plants and the control showed an upward trend, and soluble protein content decreased, while the activity of SOD, POD, CAT and APX rose first, then fell down. But in the process of salt stress, the conductivity and MDA of transformed seedlings were less than that of the control, while soluble protein content was higher than that of the control, and had higher activity than non-transformed seedlings. The analysis showed that the difference reached a significant level, and the transformed seedlings demonstrated o
bviously strong salt-resistance. |
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