| Bioinformatics analysis of ribosomal protein gene of JWB phytoplasma in the northwestern arid regions |
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| DOI：10.7606/j.issn.1000-7601.2010.03.48 |
| Key Words: jujube witches' broom phytoplasma ribosomal protein gene sequence bioinformatics |
| Author Name | Affiliation | | WU Kuan | College of Information Science and Technology, Xi'an, Shaanxi 710069, China | | YAN Yongjie | College of life Sciences, Norhwest Universiy, Xi'an, Shaanxi 710069, China | | WANG Haini | College of Plant Protection/ Shaanxi Key Laboralory of Molecular Biology for Agriculure, Northwest A & F University , Yangling, Shoanxi 712100, China | | ZHANG Jue | College of Plant Protection/ Shaanxi Key Laboralory of Molecular Biology for Agriculure, Northwest A & F University , Yangling, Shoanxi 712100, China |
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| Abstract: |
| Jujube witches' broom (JWB) is a destructive disease caused by phytoplasma , that happened almost in every cultural regions at home and abroad , bringing large economic loss. The ribosomal protein (p) gene is cloned by polymerase chain reaction from the samples of JWB which were collected from Shaanxi, Ningxia and Gansu. After se-quenced, it is found that p gene is 1199 bp, which included partial rps19, rpl22 and rps3. rpl22 and rps3 coded 129 and 252 amino acids respectively, and these two genes are not overlapped . The homologic analysis shows that pathogens from Shaanxi, Ningxia and Gansu are same; and it is similar in character among JWB, cherry lethal yellows and peach yellows isolate PY-In phytoplasmas . This study reports rp sequences of JWB phytoplasmas for the first time and identi-fies that JWB phytoplasma belongs to 16S rV-B, which can provide theory basis for research of the functional sec system of JWB phytoplasma. |
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