| Analysis of peroxidase isoenzymes from different callus of Glycyrrhiza uralensis |
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| DOI：10.7606/j.issn.1000-7601.2010.04.23 |
| Key Words: Glycrrhiza uralensis callus peroxidase isoenzyme |
| Author Name | Affiliation | | CAO Junmai | College of Biological Science and Engineering, North University for Nationalities，Yinchuan，Ningxia 750021, China | | QI Jintao | College of Biological Science and Engineering, North University for Nationalities，Yinchuan，Ningxia 750021, China | | LIU Jianli | College of Biological Science and Engineering, North University for Nationalities，Yinchuan，Ningxia 750021, China | | YAN Xingfu | College of Biological Science and Engineering, North University for Nationalities，Yinchuan，Ningxia 750021, China | | LI Man | College of Biological Science and Engineering, North University for Nationalities，Yinchuan，Ningxia 750021, China |
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| Abstract: |
| The electrophoretic patterns of peroxidase isoenzymes and peroxidase activity of callus inducted from the
rhizome, bud and leaf of Glycyrrhiza uralensis were analyzed with PAGE and spectrophotometry. The results demonstrated that the electrophoretic pattens of peroxidase isoenzymes from different callus were divided into A, B and C regions, in which the regions of A and C were the most active. All had 3 kinds of patterns and 6 bands. Meanwhile, the order of peroxidase activity from deferent callus were leaf >buds>rhizome. But too high peroxidase activity was not beneficial to the development of callus. P2 and P6 were common bands in 3 kinds of explants, while P1, P3，P4 and P5 were differ-ent bands. These could be used to identify Glycyrhiza uralensis from other species and to study the diferentiation and redifferentiation of Glycyrrhiza uralensis. |
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