| Study on physiological and biochemical characters and numerical taxonomy of rhizobia isolated from Lespedeza floribunda in Shangluo Region of Shaanxi |
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| DOI：10.7606/j.issn.1000-7601.2010.05.18 |
| Key Words: Lespedeza floribunda rhizobia phenotypic diversity |
| Author Name | Affiliation | | JI Yuliang | Department of Biological and Medical Engineering, Shangluo University, Shangluo, Shaanxi 726000, China
College of Life Sciences, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling, Shaanxi 712100, China | | ZHAN Junpeng | College of Life Sciences, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling, Shaanxi 712100, China | | WEI Gehong | College of Life Sciences, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling, Shaanxi 712100, China |
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| Abstract: |
| 59 unknown rhizobia strains isolated from Lespedeza floribunda in Shangluo, Shaanxi Province and 15 reference strains were analyzed with 125 phenotypic features and numerically classified. The result showed that the unknown strains could use the majority of all the carbn and nitrogen sources and adapt to a wide range of temperature and pH. The proportion of unknown strains which could be resistant to antibiotic, dye and heavy metals and could produce oxidase was relatively high. Some strains had endurance property to high concentrations of salt or alkali, in which about 67% strains could be resistant to
3.0% NaCl and 25% strains could grow in YMA medium with pH12. Numerical taxonomy showed that, in addition to strain CCNWSX0922, other test strains were clustered into 8 clusters at 80.5% similarity level. Clusters Ⅰ contained 6 unknown strains and 3 reference strains of Mesorhizobium. Cluster Ⅱ had 19 unknown strain
s and 6 reference strains of Sinorhizobium. Cluster Ⅲ consisted of 1 unknown strain and 3 reference strains of Rhizobium. Cluster Ⅳ and Ⅴall included reference strains of Rhizobium. Clusters Ⅵ, Ⅶ and Ⅷ may be new groups, which were not clustered into the reference strains, and their taxonomic status must be confirmed by further measurements. |
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