Identification of endophytes from Glycyrrhiza uralensis in Ningxia and screening of antagonism against pathogens of post-harvest muskmelon |
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DOI:10.7606/j.issn.1000-7601.2011.01.46 |
Key Words: Glycyrrhiza uralensis endophyte identification pathogens of post-harvest nuskmelon selection |
Author Name | Affiliation | LIU Jianli | College of Biological Sciences and Engineering, North University for Ethnics, Yinchuan, Ningxia 750021, China | CAO Junmai | College of Biological Sciences and Engineering, North University for Ethnics, Yinchuan, Ningxia 750021, China | MA Wenping | College of Biological Sciences and Engineering, North University for Ethnics, Yinchuan, Ningxia 750021, China |
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Abstract: |
The study was aimed at solating and identifying endophytes from Glycyrrhiza uralensis and detecting antimicrobial activity of these strains. The endophytes was isolated by using tissue isolation method from the root, stem and leaf of Glycyrrhiza uralensis in Ningxia and identified preliminarily based on morphological characteristics. With the pathogens of post-harvest muskmelon as test fungi,the antimicrobial activity of endophytic bacteria was investigated. The results showed that 287 endophytes were isolated from the roots, stems and leaves of Glycyrrhiza uralensis in Ningxia respectively, of which 142 were from roots, 78 from stems and 67 from leaves, and 151 belonged to bacteria and 131 to fungi. The 53 spore-producing fungi were classfied into 6 genera under 4 families and 2 orders, e.g Penicillium, Alternaria and Aspergillus were dominant. G+ and Baci
llus were dominant in endophytic bacteria. And 8 endophytic bacteria with higher activity against all pathogens of post harvest muskmelon were selected out. |
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