| Effect of low temperature freezing injury on physiological and biochemical characteristics of Vicia faba at the seedling stage and RAP-PCR fingerprint analysis |
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| DOI：10.7606/j.issn.1000-7601.2011.04.02 |
| Key Words: cold stress freezing injury RAP-PCR Vicia faba |
| Author Name | Affiliation | | CHEN Zhiyuan | College of Sciences, Northwest A & F University, Yangling, Shaanxi 712100, China
College of life Science, Northwest A & F University, Yangling, Shaanxi 712100, China | | WANG Guodong | College of Sciences, Northwest A & F University, Yangling, Shaanxi 712100, China | | DI Lijun | College of Sciences, Northwest A & F University, Yangling, Shaanxi 712100, China | | WU Yongjun | College of life Science, Northwest A & F University, Yangling, Shaanxi 712100, China | | YANG Jiaye | College of life Science, Northwest A & F University, Yangling, Shaanxi 712100, China |
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| Abstract: |
| Protein content, proline, reduced glutathione, malondialdehyde of Vicia faba at seedling stage were measured in 0℃, RAP-PCR fingerprint analysis was conducted and chilling damage-resistance gene segments were cloned. The results showed that proline increased with the time at 0℃, reduced glutathione decreased with th
e time, protein content increased first but decreased then, and malondialdehyde stayed at a steady level after freezing injury. Eight chilling damage-resistance genes were identified with 8 random primers and RAP-PCR, and four of them were successfully cloned. |
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