| Recovery effect of re-watering condition on transgenic sweet potato after drought stress and the effect of pre-spray leaves with H2O2 |
|
View Fulltext View/Add Comment Download reader |
| |
| DOI：10.7606/j.issn.1000-7601.2011.04.03 |
| Key Words: sweet potato transgenic drought stress and re-watering hydrogen peroxide |
| Author Name | Affiliation | | DONG Yonghui | State Key Laboratory of Soil Erosion and Dryland Faming on Loess Plateau/College of Life Sciences, Northwest A & F University, Yangling, Shaanxi 712100, China | | DENG Xiping | State Key Laboratory of Soil Erosion and Dryland Faming on Loess Plateau/College of Life Sciences, Northwest A & F University, Yangling, Shaanxi 712100, China | | KWAK Sangsoo | Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Korea |
|
| Hits: 136 |
| Download times: 112 |
| Abstract: |
| In the experiment, the recoverability of the sweet potato, which expressed both Cu/Zn superoxidedismutase (SOD) and ascorbate peroxidase (APX) in chloroplasts, was compared with its control plants, and foliar application of exogenous hydrogen peroxide (H2O2) before drought stress on enhancement of seedling recovery capability after drought stress was studied. The transgenic sweet potato and its non transgenic control plants were treated with 10% PEG and pretreated with 1.0 mmol／L H2O2 before drought stress, anti-oxidative enzymes system, content of MDA and H2O2 and photosynthetic rate of each plant were tested in this experiment. Under water stress and a short time after re-watering, for transgenic sweet potatothe, activities of the three mentioned anti-oxidative enzymes and photosynthetic rate were higher and the content of MDA and H2O2 were lower compared with the non-treatments; At the terminal of re-watering, the recovery capabilities of the anti-oxidative enzymes were stronger, the content of MDA and H2O2 was lower, and photosynthetic rate was higher. It was also observed that in the recovery process of the plants treated with 1.0 mmol／L H2O2 before drought stress, compared with the non-treatments, anti-oxidative enzymes was stronger, and the content of MDA and H2O2 was higher, while the photosynthetic rate was lower. A stronger drought-resistant ability and a stronger recovery capability after stress was showed in transgenic sweet potato compared with non-transgenic sweet potato under the treatment of drought stress. However, the pretreatment with 1.0 mmol／L H2O2 enhanced stress level. |
|
|
|