| Analysis of stress resistance and phylogenesis of rhizobia isolated from Caragsana spp. |
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| DOI：10.7606/j.issn.1000-7601.2011.04.41 |
| Key Words: Caragsana spp. rhizobia stress resistance phylogenesis |
| Author Name | Affiliation | | DAI Jinxia | School of Life Science, Ningxia University, Yinchuan, Ningxia 750021, China | | WANG Yujiong | School of Life Science, Ningxia University, Yinchuan, Ningxia 750021, China | | GUO Jingjing | School of Life Science, Ningxia University, Yinchuan, Ningxia 750021, China | | WU Xuejuan | School of Life Science, Ningxia University, Yinchuan, Ningxia 750021, China | | ZHANG Xiaoling | School of Life Science, Ningxia University, Yinchuan, Ningxia 750021, China |
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| Abstract: |
| Several biochemical characteristics of 62 rhizobia isolated from Caragsana spp. in Baijitan National Nature Reserve in Ninaxia were detected, including resi
stance to salt, acid-alkali, temperature variation and intrinsic antibiotics. The results indicated that 70.8% strains could tolerate NaCl stress at 4% concentration. All of the strains could tolerate Amp stress at 100 μg/mL as well as could grow at a range of pH from 5～11. Strains CNXE4, CNXLW24 and CNXC3 had high resistance, they could grow at pH from 4～12，could tolerate 6% concentration NaCl, 50 μg/mL KM and SM, 300 μg/mL AMP, and could grow after being exposed to 60℃ for 10 min. The methods of 16S rDNA PCR-RFLP and 16S rDNA sequencing were used to analysis the diversity and phylogenesis of 62 rhizobia strains. The results revealed that all the tested strains belonged to the genus of Rhizobium, Sinorhizobium, Agrobacterium, Phyllobacterium and Bradyrhizobium. The rhizobia isolated from Caragsana spp. showed a rich genetic diversity. |
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