| Cloning of a DREB gene from foxtail millet(Setaria italica L.) and its expression during drought stress |
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| DOI：10.7606/j.issn.1000-7601.2011.05.13 |
| Key Words: foxtail millet DERB transcription factor drought and re-watering express profile phylogenetic analysis1 |
| Author Name | Affiliation | | YANG Xiwen | College of Agriculture, Northwest A & F University, Yangling, Shaanxi 712100, China | | HU Yingang | College of Agriculture, Northwest A & F University, Yangling, Shaanxi 712100, China
Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China |
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| Abstract: |
| A DREB gene was amplified from Foxtail millet by PCR using a pair of primers designed on the sequences of Triticum aestivum TaDREB6 gene, the foxtail millet DREB gene was 1 113 bp in length, encoding 259 amino acids with basic amino acid regions possible nuclear location sequences(NLSs) and a highly conserved AP2/EREBP domain in the encoded putative protein. Multiple alignment analysis b
ased on the amino acids encoded by DREB genes in plants showed that the Foxtail millet DREB gene was a member of DREB2 transcription factor family, and designed as SiDREB2. The expression patterns of SiDREB2 during drought stress were investigated by means of semi-quantitative RT-PCR. The results showed that the expression patterns of SiDREB2 during drought stress was up-regulated, which suggested that it was involved in the response of foxtail millet to drought stress and might be one of the key genes for drought tolerance and water use efficiency of foxtail millet. The cloning of this gene may provide the potential for its utilization in the improvement of drought tolerance and water use efficiency in other plants. |
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