| Cloning and expression analysis of two novel drought tolerance genes coding glycine-rich RNA-binding proteins in Malus plants |
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| DOI：10.7606/j.issn.1000-7601.2011.05.14 |
| Key Words: Malus glycine-rich RNA-binding protein drought stress gene expression |
| Author Name | Affiliation | | WANG Shuncai | College of Life Science and Chemistry, Tianshui Normal University, Tianshui, Gansu 748100, China
College of Horticulture, Northwest A & F University, Yangling, Shaanxi 712100, China | | LIANG Dong | College of Horticulture, Northwest A & F University, Yangling, Shaanxi 712100, China | | SHI Shouguo | College of Horticulture, Northwest A & F University, Yangling, Shaanxi 712100, China | | MA Fengwang | College of Horticulture, Northwest A & F University, Yangling, Shaanxi 712100, China | | SHU Huairui | College of Horticulture, Northwest A & F University, Yangling, Shaanxi 712100, China |
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| Abstract: |
| Two Malus (apple) drought-related genes, MpGR-RBP1 (HM042682) and MhGR-RBP1 (HQ380209), were successfully identified from the drought stress-treated Malus prunifolia and M. hupehensis, respectively, using library screening, in silico cloning and RT-PCR. The proteins of two homologous genes, MpGR
-RBP1 and MhGR-RBP1, were consisted of 171 and 164 amino acids, respectively. Their deduced amino acids contain a N-terminal RNA recognition motif (RRM
) and a C-terminal glycine-rich domain, a structure which was found in stress-induced GR-RBP protein family in other plants. Bioinformatics analysis confirmed that both MpGR-RBP1 and MhGR-RBP1 proteins belong to the plant GR-RBP family, members of which play important roles in post transcriptional regulation of gene expression under various stress conditions. The expression profiles of the
two apple GR-RBP transcripts were detected by quantitative real-time RT-PCR. MpGR-RBP1 and
MhGR-RBP1 were expressed in various plant tissues including roots, shoots, and leaves. Both MpGR-RBP1 and MhGR-RBP1 were up-regulated under drought stress, with expression levels in the former being higher than in the latter. These results indicated that MpGR-RBP1 and MhGR-RBP1 might be involved in apple plants response to drought stress, and it suggested that differential configuration of drought-tolerant proteins may be contributed to capability of drought resistance in apple plants. |
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