| Isolation and identification of inorganic phosphorus-solubilizing bacteria by Biolog and molecular method |
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| DOI：10.7606/j.issn.1000-7601.2012.01.32 |
| Key Words: inorganic phosphate solubilizing bacteria identification Biolog 16S rDNA |
| Author Name | Affiliation | | LIU Jiang | College of Sciences, Northwest A & F University, Yangling 712100, China | | GU Jie | College of Resources and Environment, Northwest A & F University, Yangling 712100, China | | GAO Hua | College of Resources and Environment, Northwest A & F University, Yangling 712100, China | | QIN Qingjun | College of Resources and Environment, Northwest A & F University, Yangling 712100, China | | CHEN Shengnan | College of Resources and Environment, Northwest A & F University, Yangling 712100, China | | LI Hailong | College of Resources and Environment, Northwest A & F University, Yangling 712100, China | | ZHANG Weijuan | College of Resources and Environment, Northwest A & F University, Yangling 712100, China | | GUO Xingliang | College of Sciences, Northwest A & F University, Yangling 712100, China | | ZHANG Hongbin | College of Resources and Environment, Northwest A & F University, Yangling 712100, China | | CHEN Lin | College of Sciences, Northwest A & F University, Yangling 712100, China | | SHAO Li | College of Sciences, Northwest A & F University, Yangling 712100, China |
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| Abstract: |
| A study was conducted to isolate effective inorganic phosphorus-solubilizing bacteria for potential research and production of biofertilizer. Three strains, named as NO.9, NO15 and NO.17, are isolated from soi
l samples collected from Qinling Mountains by their dissolving phosphorus circle on PKO Agar media. After 5 days incubation, through Mo-Sb colorimetry, the soluble phosphorus content in liquid media is 98.12 mg/L, 80.37 mg/L and 104.91 mg/L respectively, and the content of microbial biomass phosphorus is 62.85 mg/L, 53.61 mg/L and 36.59 mg/L. Analysed by physiology and biochemistry experiments, Biolog and 16S rDNA sequencing reflects that all of the three strains are gram-negative intestinal bacteria. and NO.9 strain is identified as Inquilinus ginsengisoli, NO.15 strain as Acinetobacter calcoaceticus and NO.17 strain as Pseudomonas fluorescens. |
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