Isolation and identification of inorganic phosphorus-solubilizing bacteria by Biolog and molecular method
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DOI:10.7606/j.issn.1000-7601.2012.01.32
Key Words: inorganic phosphate solubilizing bacteria  identification  Biolog  16S rDNA
Author NameAffiliation
LIU Jiang College of Sciences, Northwest A & F University, Yangling 712100, China 
GU Jie College of Resources and Environment, Northwest A & F University, Yangling 712100, China 
GAO Hua College of Resources and Environment, Northwest A & F University, Yangling 712100, China 
QIN Qingjun College of Resources and Environment, Northwest A & F University, Yangling 712100, China 
CHEN Shengnan College of Resources and Environment, Northwest A & F University, Yangling 712100, China 
LI Hailong College of Resources and Environment, Northwest A & F University, Yangling 712100, China 
ZHANG Weijuan College of Resources and Environment, Northwest A & F University, Yangling 712100, China 
GUO Xingliang College of Sciences, Northwest A & F University, Yangling 712100, China 
ZHANG Hongbin College of Resources and Environment, Northwest A & F University, Yangling 712100, China 
CHEN Lin College of Sciences, Northwest A & F University, Yangling 712100, China 
SHAO Li College of Sciences, Northwest A & F University, Yangling 712100, China 
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Abstract:
      A study was conducted to isolate effective inorganic phosphorus-solubilizing bacteria for potential research and production of biofertilizer. Three strains, named as NO.9, NO15 and NO.17, are isolated from soi l samples collected from Qinling Mountains by their dissolving phosphorus circle on PKO Agar media. After 5 days incubation, through Mo-Sb colorimetry, the soluble phosphorus content in liquid media is 98.12 mg/L, 80.37 mg/L and 104.91 mg/L respectively, and the content of microbial biomass phosphorus is 62.85 mg/L, 53.61 mg/L and 36.59 mg/L. Analysed by physiology and biochemistry experiments, Biolog and 16S rDNA sequencing reflects that all of the three strains are gram-negative intestinal bacteria. and NO.9 strain is identified as Inquilinus ginsengisoli, NO.15 strain as Acinetobacter calcoaceticus and NO.17 strain as Pseudomonas fluorescens.