| Agrobacterium-mediated transformation of RNAi vector with ACO gene on anti-aging of lily |
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| DOI：10.7606/j.issn.1000-7601.2012.02.16 |
| Key Words: Agrobacterium-mediated ACO gene RNAi expression vector anti-aging lily |
| Author Name | Affiliation | | LI Xiaoling | Department of Biomedical Engineering, Shangluo University, Shangluo, Shaanxi 726000, China | | LIU Yali | College of Horticulture, Northwest A & F University, Yangling, Shaanxi 712100, China | | HUA Zhirui | Department of Biomedical Engineering, Shangluo University, Shangluo, Shaanxi 726000, China |
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| Abstract: |
| With the scale of asiatic lily as explants, the RNAi vector with ACO anti-aging gene was transferred into lily with Agrobacterium mediated transformation to study the factors which affected the transformation and to provide theoretical an
d technical basis for anti aging of plants. The results showed that the highest transformation efficiency was obtained through the following transformation procedure: after preculture for 3 d, 25 mg/L acetosyringone (AS) was added to Agrobacterium and co-cultivation medium respectively, the explants were inoculated for 8min with diluted OD600=0.8 recombinant Agrobacterium tumefacien culture liquid, and then co-cultivated for 3 d. PCR detection showed that some resistant plants were positive, which indicated preliminarily that the ACO gene was integrated into the genome of transgenic plants. |
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