| Amount distribution and category characteristics of endogenousrhizobium in Amorpha fruticosa seeds |
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| DOI：10.7606/j.issn.1000-7601.2012.04.41 |
| Key Words: seed of Amorpha fruticosa endogenous rhizobium amount distribution category characteristics |
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| Abstract: |
| To determine the amount distribution and category characteristics in seed capsule, pericarp, cotyledon and embryo of rhizobium in Amorpha fruticosa seeds, amount and category of endogenous rhizobium in different parts seed of Amorpha fruticosa were analyzed. The result indicated that there was significant discrepancy in amount distribution and category characteristics in endogenous rhizobium. Most endogenous rhizobia were distributed in seed caps
ule (6.663×106 cfu／pod), which accounted for 50 percent of the total strains, and then pericarp (3.335×106 cfu／pod). Only small amount of endogenous rhizobium was present in cotyledon and embryo (2.087×106 cfu／pod, 1.250×106 cfu／pod). 32 strains of rhizobium, isolated from different parts seed of Amorpha fruticosa, were divided into 5 types based on the characteristic maps of RAPD-PCR groups (A, B, C,D and E) . Most of the stains were B type, accounting for 40.62% of the total. 8 strains belonged to C type, and the number of stains belong
ing to D, A and E types were 6, 4 and 1. There were 5 groups in seed capsule of 16 strains, 3 groups in pericarp and only 3 species of cotyledon and embryo. These showed the partiality and diversity of endogenous rhizobium in different parts of seed of Amorpha fruticosa with amount distribution position and category characteristics. |
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