Isolation and preliminary identification of endophytic fungi from Sophora alopecuroides in Ningxia
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DOI:10.7606/j.issn.1000-7601.2012.05.36
Key Words: Sophora alopecuroides  endophytic fungus  isolation  preliminary identification
Author NameAffiliation
LIU Jianli College of Biological Sciences and Engineering, North University for Ethnics, Yinchuan, Ningxia 750021, China 
LIN Qin College of Biological Sciences and Engineering, North University for Ethnics, Yinchuan, Ningxia 750021, China 
YAN Xingfu College of Biological Sciences and Engineering, North University for Ethnics, Yinchuan, Ningxia 750021, China 
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Abstract:
      In order to investigate the diversity of endophyteic fungi in Sophora alopecuroides in Ningxia, the endophytic fungi was isolated using tissue isolation method from the root, stem and leaf of Sophora alopecuroides in Ningxia and identified preliminarily based on morphological characteristics in this paper. 115 endophytic fungi were isolated from 875 sterilized tissues of Glycyrrhiza uralensis in Ningxia, of which 18 isolated from roots, 41 isolated from stems, 56 isolated from leaves, which account for 15.7%, 35.7% and 48.6% of total endophytic fungi respectively. The result of preliminary identification showed that the 113 fungi that produced spores were classified to 7 genera under 5 families, 3 orders and 2 classes. 3 belongs to Penicillium, accounting for 2.6%; 4 belongs to Periconia, accounting for 3.6%; 45 belongs to Alternaria, accounting for 39.1%; 9 belongs to Fusarium, accounting for 7.8%; 10 belongs to Aspergillus, accounting for 8.7%; 30 belongs to Dendrophoma, accounting for 26.1%; 9 belongs to Rhizopus, accounting for 7.8%. But Alternaria and Dendrophoma were dominant. It was concluded that the entophytes in Sophora alopecuroides in Ningxia were diversity and rich, and there were some differences at quantity and species in different organs of Sophora alopecuroides in Ningxia.