| Analysis of self-compatibility genes by mRNA differential display technique and inheritance of self-compatibility in E.sativa Mill |
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| DOI：10.7606/j.issn.1000-7601.2012.06.04 |
| Key Words: E. sativa Mill inheritanee of self-compatibility self-compatible genes mRNA differential display |
| Author Name | Affiliation | | FAN Huiling | College of Agriculture and Biotechnology, Hexi University, Zhangye, Gansu 734000, China | | BAI Shengwen | College of Agriculture and Biotechnology, Hexi University, Zhangye, Gansu 734000, China | | ZHANG Fenqin | College of Agriculture and Biotechnology, Hexi University, Zhangye, Gansu 734000, China | | XIAO Zhanwen | College of Agriculture and Biotechnology, Hexi University, Zhangye, Gansu 734000, China | | CHEN Xiubin | College of Agriculture and Biotechnology, Hexi University, Zhangye, Gansu 734000, China | | SUN Wancang | Gansu Agricultural University, Lanzhou, Gansu 730070,
China |
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| Abstract: |
| 18 hybridized combinations were made between 8 self-compatible lines and 4 self-incompatible lines, and inheritance of self-compatibility in E.sativa Mill was analysed. Furthermore, expresstion tissue of self-compatible genes was preliminary analysed, and specific cDNA fragments related to self-compatibility were isolated and screened by using mRNA differential display technique. The results showed that all the F1 plants were self-incompatible, and the F2 and BC1 population segregated in ratio. Through chi square test, the segregation ratios of self-incompatibility to self-compati-bility in F2 and BC1 population were coincided with the ratio of 1:3 and 1:1, respectively. Therefore, it was inferred that the hereditary of self-compatibility in Yunjie was controlled by one pair recessive nucleus gene. Self-compatible genes of Yunjie was not constitutive expression, but that belonged to specific tissue expression. Self-compatible lines had 3 differ-entially expressed bands which exhibited stably with different primers, these cDNA bands were closely related to self-eompatibility of Yunjie. |
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