| Molecule-pyramiding breeding of new improved wheat varieties by introducing sorghum DNA via pollen-tube pathway |
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| DOI：10.7606/j.issn.1000-7601.2013.02.02 |
| Key Words: wheat sorghum DNA pollen-tube pathway HMW-GS stripe-rust resistance molecule-pyramiding breeding. |
| Author Name | Affiliation | | OU Qiaoming | Bio-technology Institute, Gansu Academy of Agricultural Scie
nces, Lanzhou, Gansu 730070, China | | CUI Wenjuan | Bio-technology Institute, Gansu Academy of Agricultural Scie
nces, Lanzhou, Gansu 730070, China | | WANG Wei | Bio-technology Institute, Gansu Academy of Agricultural Scie
nces, Lanzhou, Gansu 730070, China | | NI Jianfu | Bio-technology Institute, Gansu Academy of Agricultural Scie
nces, Lanzhou, Gansu 730070, China | | WANG Hongmei | Bio-technology Institute, Gansu Academy of Agricultural Scie
nces, Lanzhou, Gansu 730070, China | | YANG Fangping | Bio-technology Institute, Gansu Academy of Agricultural Scie
nces, Lanzhou, Gansu 730070, China | | LUO Junjie | Bio-technology Institute, Gansu Academy of Agricultural Scie
nces, Lanzhou, Gansu 730070, China |
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| Abstract: |
| The genomics DNA of sorghum was introduced via the pollen tube pathway into the strains of soft-grain wheat that were highly infectable to stripe rust. Two stable variants was obtained with the aid of the key
technologies including culturing of young embryo and screening of early generation variation, and the apparent variation of biological characteristics, such as stripe-rust resistance, quality indicators, HMW-GS, alleles in Gul D1 loci an
d peroxidase, was displayed in the new variants compared with their receptors. This showed that the introduction of sorghum genomics DNA caused the changes of gene expression in wheat strains, and their regional adaptabilities were also dif
ferent from their receptors accordingly. Therefore, the genetic transformation of the traits of stripe-rust resistance and HMW-GS from sorghum into wheat was realized, and the objective of improving target characters, such as biological indicators, stripe-rust resistance, yield and quality, was achieved. It was speculated that the above results might be led by the genetic transformation of stripe-rust resistant gene from sorghum and biological mutation of HMW-GS gene as well as polygenic pyramiding. The variation of alleles in Gul D1 loci of HMW-GS co
uld be attributed to the biological mutagenesis, and the variation of stripe-rust resistance could be ascribed to the target-oriented transformation of genomic DNA from sorghum. |
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