Physiological responses of Vicia faba L.leaves to salt-alkali stress
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DOI:10.7606/j.issn.1000-7601.2013.03.26
Key Words: Vicia faba L.  leaf  salt-alkali stress  physiological responses
Author NameAffiliation
QIAO Feng Education Ministry Key Lab of Environment and Resource in Qinghai-Tibet Platean, Qinghai Normal University, Xining, Qinghai 810008, China 
GENG Guigong Institute of Crop Breeding and Cultivation, Qinghai Academy of Agriculture and Forestry, Xining, Qinghai 810016, China 
CHEN Zhi Education Ministry Key Lab of Environment and Resource in Qinghai-Tibet Platean, Qinghai Normal University, Xining, Qinghai 810008, China 
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Abstract:
      In attempt to study physiologic responses of Vicia faba L.to salt-alkali stress, seedlings of Vicia faba L. were treated with three different eombinations of salts, KCl/NaCl, KCl/Na2CO3,KCI/Na2SO4, for 2 weeks. The fresh weight of seedlings and malondialdehyde(MDA) were then measured, and free proline contents and antioxidant enzymes activity of leaf were further analyzed. The results indicated that with extensions of stress time, malondialdehyde(MDA) and free proline contents were increased, while the freshweight of seedlings was increased at first and then decreased. Free proline contents increased sharply at 11 days of stress. Activities of four antioxidant enzymes, superoxide disrutase(SOD), peroxidase(POD), catalase(CAT), ascorbate peroxidase(APX), were increased at first, were then decreased. At 11 days of stress, order of the activity increments of these four antioxidant enzymes was SOD>CAT>APX or POD. In conclusion, SOD played critical role in the response of Vicia faba L. to salt-alkali stress, and free proline an important osmotic substance.