| The effect of hexaconazole-Cu complex on proline accumulation in wheat seedlings under NaCl stress |
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| DOI：10.7606/j.issn.1000-7601.2013.04.020 |
| Key Words: hexaconazole-Cu complex proline semiquantitative RT-PCR salt stress |
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| Abstract: |
| The hexaconazole-Cu complex, synthesized by hexaconazole and copper acetate, was used to soak the seed of wheat to ameliorate the wheat seedlings salinity stress induced by sodium chloride (NaCl). The effect of hexaconazole-Cu complex on the anti-salinity stress of wheat seedlings was evaluated by proline accumulation level and the gene expression level of Δ1-pyrroline-5-carboxylate synthetase (
P5CS) and Δ1-pyrroline-5-carboxylate reductase (PC5R) of wheat seedlings. The results showed that in the presence of 200 mmol·L-1 NaCl, the proline content of the 0.03 mmol·L-1 complex treated wheat seedlings was 55.44 μg·g-1, which was 13.68% higher than that in hexaconazole treatment, and 58.45% higher than that in copper acetate treatment. The gene expression levels of P5CS and P5CR were quantitated by semiquantitative RT-PCR analysis. The results showed that the hexaconazole-Cu complex enhanced the transcript levels of P5CR and P5CS. After 6 h treatment of the complex in the presence of 100 mM NaCl, the P5CS express level reached the maximum, and was significantly higher than in hexaconazole treatment. It was obvious that the hexaconazole-Cu complex enhanced the proline contents, thereby played a protective role in the salinity stress of wheat seedlings. |
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