| Effects of different concentrations of chemical agent GSC on main |
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| DOI：10.7606/j.issn.1000-7601.2013.04.025 |
| Key Words: winter rapeseed (Brassica rapa) chemical hybridizing agent GSC male sterility |
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| Abstract: |
| Using the cultivar Jian 94 as a material, the effects of different concentrations of the chemical agent GSC on the main traits of winter rapeseed (Brassica rapa) were studied. The results showed: GSC treatment caused a negative effect on plant growth, but there was no significant effec
t on seed setting; the growth of corolla and petals was inhibited, and the corolla diameter and petal area were decreased with the increase of GSC concentration; the length of stamens was gradually shortened, while the pistils were not changed significantly; under the concentration of T7 (0.5 mg·L-1 and 0.6 mg·L-1 at first and second time, respectively), the filaments became atrophied, and the anthers became shriveled with a large number of sterile pollens. GSC
could induce male sterility of winter rapeseed, and the rate of sterile plants and dead pollens was increased with the rise of GSC concentration. Under the concentration of T7, the rate of sterile plants and dead pollens was 85.67% and 82.30%, respectively. Meanwhile, the anthers became hypogenetic, the tapetal cells disintegrated in advance or disappeared along with the degradation of pollen grains, and the spore balls stuck closely to the inner wall and their number became small. The results demonstrated that the chemical agent GSC could cause a certain effect on male sterility, and there was a relationship between sterilization rate and GSC concentration. |
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