| In the process of breeding research by ways of sel
f incompatible lines in E. Sativa Mill., there is often a phenomenon that t
he seed setting percentage from the cross of two parents is high in bud stage, b
ut the number of seeds is low in late stage owing to the natural segregation. By
using the method of complete diallel crossing, the purity of S alleles in 10 se
lf incompatible lines of E. Sativa Mill. was studied, and the expression ti
ssue of self incompatible genes was analyzed with DDRT-PCR technique. The resul
ts showed that the self compatible index of Yunjie 1, Yunjie 3, Yunjie 5, Yunji
e 6, Yunjie 8, Yunjie 9 and Yunjie 13 was below 1 after selfing or outcrossing
among these 7 self incompatible lines, that is to say, their self incompatibil
ity was stable. However, the self incompatibility of Yunjie 2, Yunjie 4 and Yun
jie 10 was not stable. DDRT-PCR analysis showed that the self incompatible gene
s of E. Sativa Mill. didn’t belong to constitutive expression, but to speci
fic tissue expression. Therefore, when creating F1 hybrids by ways of self inco
mpatible lines, it is necessary for us to determinate the purity of S alleles an
d their genotypes besides agronomic traits and self incompatible stability. |