Three base nitrogen fertilizer levels, including 0
, 1.5 kg·hm-2 and 3.0 kg·hm-2, were adopted in a pot experiment to
study the changes of enzyme system of carbon nitrogen metabolism in leaves an
d pods of kidney bean (Phaseolus vulgari L.). The results showed that, after
the formation of pods, the activity of phosphoenolpyruvate carboxylase (PEP), p
olyphenol oxidase (PPO), superoxide dismutase (SOD) and catalase (CAT) in pods g
radually increased with the increase of nitrogen. Under the same nitrogen level,
the activity of sucrose synthase (SS), sucrose phosphate synthase (SPS), nitrat
e reductase (NR) and PEP in leaves was greater than that in pods, while the cont
ent of total soluble sugar and protein in pods was higher than that in leaves. T
he activity of PEP in leaves was correlated significantly with the activity of S
PS and NR in pods. It could be concluded that, in the plants of kidney bean, the
source organ was the active center of metabolism, while the sink organ was the
growth center, and the carbon nitrogen metabolism process of the latter was mai
nly regulated by the former. |