Effects of different priming treatments on germination and seedling drought resistances of Astragalus membranaceus (Fisch) Bge.
View Fulltext  View/Add Comment  Download reader
  
DOI:10.7606/j.issn.1000-7601.2013.05.039
Key Words: Astragalus membranaceus (Fisch.)Bge.seed  priming treatments  permination  drought resistance
Author NameAffiliation
HE Jun1, PU Jun2, WANG Wei-ling2, GAO Qing-ge2 (1.商洛学院生物医药工程系 陕西 商洛 726000 2.西北农林科技大学生命科学学院 陕西 杨凌 712100) 
Hits: 2422
Download times: 1555
Abstract:
      Objective: Physiological effects of seed priming on drought resistance of Astragalus membranaceus (Fisch.) Bge. seedlings were studied in this paper. Method: After Astragalus membranaceus (Fisch.) Bge.seeds were subject to different priming with H2O, 20% PEG-6000,0.25% KNO3,100 mg·L-1 GA3 and 12.5 mg·L-1 6-BA respectively for germination experiment, seedlings were under 20% PEG-6000 simulated drought stress to measure d rought related physiological indicators. Results: The sequence of Germination energy and germination percentage was identical: 12.5 mg·L-1 6-BA>100 mg·L-1 GA3>0.25% KNO3>20% PEG-6000>H2O>CK; and the sequence of Simplified vigor index was 0.25% KNO3>100 mg·L-1 GA3≈20% PEG-6000>H2O>12.5 mg·L-1 6-BA>CK. Malondialdehyde (MDA) content, soluble protein content, proline content, chlorophyll content, superoxide dismutase (SOD), peroxidase(POD) and catalase activity (CAT) of seedling have a significant difference between CK.According to comparing the Average value of subordinate function, the rank of drought resistance was H2O>20% PEG-6000>0.25% KNO3≈12.5 mg·L-1 6-BA>CK>100 mg·L-1 GA3. Conclusion: 20% PEG-6000, or 0.25% KNO2 can effectively promote seed germination and improve seedling drought resistance.