Effects of seed ultra-drying storage on growth and physiological characteristics of Medicago sativa seedlings
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DOI:10.7606/j.issn.1000-7601.2014.01.028
Key Words: Medicago sativa L.  ultra-dried seed  seedlings  physiology
Author NameAffiliation
HUO Ping-hui, LI Jian-feng, SHI Shang-li, ZHANG Shu-qing (1.甘肃农业大学 草业学院 甘肃 兰州 730070 2.草业生态系统教育部重点实验室/中-美草地畜牧业可持续发展研究中心 甘肃 兰州 730070) 
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Abstract:
      Medicago sativa L. cv. Longdong was used as test material to explore the effect of ultra-drying storage on seedling growth. The seeds were ultra-dried from an initial moisture content of 9.03% (CK) to 7.09%~4.59%, respectively, with silica gel method, and then hermetically sealed in aluminized paper at ambient temperature for storage. One year later, all the seeds with different moisture contents were sown in pot with sand to study the growth and physiology of the resulting seedlings. The result indicated that moderately ultra-dried seeds performed better in seedling emergence, shoot height and MDA content, on day 30, seedling number of treatments with moisture contents from 6.36 to 4.59 were 25%~50% times higher than the control, and on day 60, shoo t height of treatments with moisture contents from 6.36 to 5.46 were 11.02%~15.68% times higher than control. MDA content of all the ultra-drying treatments were only 44.92%~70.29% of the CK treatment. Root volume, aboveground dry weight, underground dry weight and soluble sugar content were only 55.36%~89.29%, 63.06%~88.05%, 50.71%~76.69% and 63.35%~97.86% of the CK, respectively, ex cept the non-significant difference of 4.59% treatment in leaf number and 5.18% treatments in nodule number compared with CK All the other treatments were found significantly lower than CK in leaf and nodule numbers, and the nodule number of the ultra-dry treatments was only 63.00%~92.56% of CK, indicating that ultra-drying storage could increase seedling emergence, shoot height and plant resi stance, but decrease plant biomass and root volume.