| Identification of the genetic relationship between different cultivated varieties in Qinling Slope dryland |
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| DOI：10.7606/j.issn.1000-7601.2014.02.024 |
| Key Words: polyacrylamide gelelectrop horesis esterase transfer rate joint coefficient enzyme similarity |
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| Abstract: |
| In order to provide the basis for variety breeding and germplasm identification of Chinese traditional medicine Platycodon grandiflorum in Qinling slope dryland, platycodon esterase isozymes (EST) expression difference of different leaf position in three different regions is studied
by application of polyacrylamide gel electrophoresis (PAGE) technique, and through the EST-PAGE map mobility (Rf), joint coefficient (S) (T), enzyme spectrum similarity comprehensive analysis method, Put forward the preliminary kinship evaluation of shangluo Platycodon grandiflorum cultivation varieties in the different regions of slope dryland. The results showed that the genetic relationship between different regions size order:Pingliang-Gansu＞
Zibo-Shandong＞Guangyuan-Sichuan＞Lantian-Xian＞Chifeng-Neimenggou≈Shangluo-Shaanxi, there are no difference between Chifeng-Neimenggou and Shangluo-Shaanxi in species of the platycodonis. |
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