Effects of fermentation medium of Trichoderma aureoviride on growth and physiological characteristics of ryegrass
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DOI:10.7606/j.issn.1000-7601.2014.02.025
Key Words: Trichoderma aureoviride  growth promoting  ryegrass  physiological characteristics
Author NameAffiliation
ZHANG Shu-wu1,2,3,4, XU Bing-liang1,2,3,4, CHENG Ling-juan1 (1.甘肃农业大学草业学院 甘肃 兰州 730070 2.草业生态系统教育部重点实验室 甘肃 兰州 730070 3.甘肃省草业工程实验室 甘肃 兰州 730070 4.中-美草地畜牧业可持续发展研究中心 甘肃 兰州 730070) 
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Abstract:
      Effects of different dilutions of the fermentation medium of Trichoderma aureoviride on growth and physiological characteristics of ryegrass were studied by seed germination and seedling pot experiment in vitro and pot experiments. Results showed that different dilutions of the fermentation medium of T. aureoviride can significantly increase the rates of seed germination, germination index and vigor index, especially for the dilutions of 100 times by which seed germination rates, germination index and vigor index were increased by 96.55%, 37.41 and 7.83, respectively. Pot experiment results showed that different dilutions of the fermentation medium of T. aureovirid e had obvious effects on the growth of ryegrass seedlings, and can significantly increase the content of chlorophyll, protein, and defensive enzymes. Especially the dilutions of 100 times can increase ryegrass root length, plant height, fresh weight and dry weight by 16.95%、13.33%、40.57%、73.68%, and 36.36%, respectively. The content of chlorophyll and soluble protein were increased by 84.09% and 75.50%. Polyphenol oxidase, peroxidase and phenylalanine enzyme activity were significantly higher than control after treatment with the dilutions of 100 times of T. aureoviride. Maximum activities of POD, PPO and PAL were observed on 3 and 4 days after inoculation. Therefore, the results suggested that the fermentation medium of T. aureoviride has substantial effects on growth and physiological characteristics of ryegrass.