| In order to improve the alfalfa's resistance to drought, the drought stress was simulated on leaf callus of alfalfa that was carried by satellite, and the response to PEG stress and 6 physiological and biochemical indexes were measured. The results showed that the rate of fatality increased gradually on alfalfa callus, with half lethal concentration and lethal concentration of PEG being 25% and 30%, as PEG concentration increased. In comparison with CK, satellite carrying significantly improved the contents of soluble sugar, soluble protein, and increased the POD and SOD activity, reduced the relative conductivity and malondialdehyde content. Satellite carrying increased drought resistance of the alfalfa callus, which could be useful for obtaining alfalfa mutants with high drought resistance. |