| Evaluation of drought resistance of different habitats of Medicago ruthenica seed germination stage under PEG-6000 stress |
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| DOI：10.7606/j.issn.1000-7601.2014.05.013 |
| Key Words: Medicago ruthenica seed germination PEG stress subordinate function |
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| Abstract: |
| Drought resistances were evaluated based on seeds germination stage under different osmotic potential of polyethylene glycol (PEG 6000) among 12 accessions of Medicago ruthenicac collected from Jingtai, Longxi, Zhenyuan, Yongchang, Tianshui, Yuzhong, Ningxian, Xiahe, Linxia and Weiyuan of Gansu Prvience, Tumote of Inner Mongolia, and Longxian of Shanxi. The results showed that the germination rate, germination index and vigor index of M. ruthenica decreased, and the growth of plumule and radical were inhibited under PEG stress. The seed germination of M. ruthenica (Longxi) was improved by draught stress of -0.3 MPa osmotic potential, and the relative germination index and relative vigor index were significantly higher than other 11 materials under this concentration. The relative radical length of M. ruthenica(Jingtai) and M. ruthenica (Tumote) were higher than others under draught stress of -0.3 MPa osmotic potential, which showed some drought resistance. The growth of radical was seriously inhibited under high osmotic potential stress. Based on the relative germination rate, relative germination index, relative vigor index, relative plumule length and relative radical length, The comprehensive evaluation of 12 accessions of M. ruthenica by the subordinate function method indicated that the drought resistance ability were ranked as Jingtai＞Longxi＞Tumote＞Zhenyuan＞Yongchang＞Tianshui＞Yuzhong＞Ningxian＞Xiahe＞Linxia＞Longxian (from Shanxi）＞Weiyuan. |
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