Genetical characteristic of stay-green of flag leaf after flowering in Recombinant Inbred Lines (RILs) of wheat and its correlation analysis with grain weight under drought stress
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DOI:10.7606/j.issn.1000-7601.2014.06.010
Key Words: wheat  RILs  drought stress  chlorophyll content  thousand-grain weight  quantitative genetics
Author NameAffiliation
CHEN Xiao-ping1, YANG De-long1, LI Meng-fei1, CHENG Hong-bo1, XING Hua1, CHAI Shou-xi2, LI Wei1 (1.甘肃省干旱生境作物学重点实验室/甘肃农业大学生命科学技术学院 甘肃 兰州 730070 2.甘肃农业大学农学院 甘肃 兰州 730070) 
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Abstract:
      To investigate the quantitative genetical characteristics of SPAD of flag leaf after flowering at different sites and its correlation analysis with thousand-grain weight (TGW), a 120 recombinant inbred lines (RILs) derived from two Chinese winter wheat cultivars were used. The recipient parent Longjian 19 had strong drought resistance with lower chlorophyll content (SPAD), while the donor Q9086 showed contrasting traits. The results indicated the interaction between water and genotype. There was significantly positive correlation between SPAD of flag leaf and TGW after flowering, while higher correlation (r=0.59**~0.69**) was detected between TGW and SPAD of flag leaf (SPADg) during filling stage. The SPADg showed significantly positive effect on TGW, while that of flag leaf (SPADf) during flowering stage was opposite. Gross effect of SPAD to TGW in drought stress was significantly higher than those in normal irrigation condition, and gross effect of SPADg to TGW was higher than SPADf. Heritability of SPAD of flag leaf and TGW were generally lower (hB2=0.15~0.44) under different treatments. The number of genes controlling SPADf were 22~36 and 50~59, and those controlling SPADg were 24~25 and 31~33, while those controlling TGW were 10~11 and 13~14 under drought stress and normal irrigation condition, respectively. The result indicates the feasibility of using this RILs population in genetical study of wheat drought resistance.