| Genetic diversity analysis of tartary buckwheat and selection of core collections |
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| DOI：10.16302/j.cnki.1000-7601.2015.01.044 |
| Key Words: tartary buckwheat genetic diversity core collections SSR |
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| Abstract: |
| SSR marker was utilized in this study to analyze the genetic diversity of 210 tartary buckwheat accessions from Tibet, Shaanxi, Sichuan and Guizhou provinces. Results had indicated that among the overall 50 SSR primers, there were 16 primers demonstrating good polymorphisms. 178 bands were obtained and 66.3% of them (118) were polymorphic bands. The genetic similarity levels of 210 accessions ranged from 0.62 to 0.98, exhibiting an average value of 0.80. At the similarity level of 0.88, these tartary buckwheat varieties could be clustered into five groups. According to the ultimate dendrogram, these tartary buckwheat accessions which came from the same planting region had presented a similar clustering tendency, reflecting that the genetic information of tartary buckwheat might be affected remarkably by its geographic distribution. Moreover, 41 accessions clustered in a V group which came from Tibet (27), Shaanxi (8), and Guizhou (6) provinces showed abundant genetic diversity. Compared with other accessions, these 41 germplasms possessed not only large genetic differences, but also a wide range of genetic origins. Thereby it is reasonable to choose them as the foundation for selections of core collections in tartary buckwheat. |
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