| To improve drought resistance of crops, inducible promoter rd29A was cloned from Arabidopsis thaliana by PCR method. Sequencing analysis indicated that the cloned fragment showed 99.47% identity to the published sequence (D13044). An rd29a driving GUS expression vector pBI121-rd29-GUS was constructed by DNA recombinant technology. By Agrobacterium-mediated transformation, pBI121-rd29-GUS was transformed into tobacco. The function of rd29A promoter was identified through the expression of GUS protein in transgenic tobacco. GUS activity in transgenic tobacco leaf showed that the rd29A promoter could drive efficient expression of the target gene. rd29A promoter could be used in subsequent transgenic study of drought resistance in potato. |