Isolation of Bacillus mucilaginosus and its influence on cotton seed germination in the desert sand
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DOI:10.7606/j.issn.1000-7601.2015.06.13
Key Words: potassium bacteria  identification  cotton germination rate  superoxide dismutase  desert sand
Author NameAffiliation
WU Jiang-li 西南科技大学生命科学与工程学院 四川 绵阳 621010 生物质材料教育部工程研究中心 四川 绵阳 621010 
LUO Xue-gang 生物质材料教育部工程研究中心 四川 绵阳 621010 
LI Bao-qiang 新疆慧尔农业科技股份有限公司 新疆 昌吉 831100 
LI Zi-fan 西南科技大学生命科学与工程学院 四川 绵阳 621010 生物质材料教育部工程研究中心 四川 绵阳 621010 
YANG Sheng 西南科技大学生命科学与工程学院 四川 绵阳 621010 生物质材料教育部工程研究中心 四川 绵阳 621010 
ZHONG Xue-mei 西南科技大学生命科学与工程学院 四川 绵阳 621010 
WU Hong-li 成都学院(成都大学)期刊中心 四川 成都 610052 
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Abstract:
      The potassium bacteria obtained from collected soil samples were isolated and identified, and then the effects of different contents from viable bacteria on the cotton seed germination rate, fresh weight, shoot and root length of seeding, the content of superoxide dismutase (SOD), peroxidase (POD) and malondialdehyde (MDA) were studied using desert sands as soil matrixes. The results showed that the potassium bacteria was Bacillus mucilaginosus. When different bacterial contents in bacterial liquid were added into desert soil, germination time of cotton seeds was 0~24 hours earlier than the control experiment. Germination rate showed a tendency to become increased first and then went decreased with the addition of bacterial content, reaching a highest value of 70.29% when the bacterial content was 0.8×108 CFU·mL-1. The length of the cotton stem and taproot were relatively increased by 0.5~1.2 cm and shortened by 0.20~0.65 cm, respectively. However, the growth of lateral root was promoted, and the fresh weight of seedlings was increased by about 0.0662~0.1582 g. In the meantime, both the content of related enzymes and malondialdehyde in the seedlings became increased or decreased to some extent.